differential centrifugation

Mitochondrial purification process using the two-step differential centrifugation and density gradient method .

试验方法采用多步差速离心和密度梯度离心联合法分离纯化肝脏线粒体。

Cells by differential centrifugation and discontinuous sucrose density gradient centrifugation .

细胞捣碎后，通过差速离心和不连续蔗糖密度梯度离心获得富集液泡膜的膜微囊。

Wheat leaf mitochondria are isolated with differential centrifugation .

用差速离心法分离绿色小麦叶片线粒体。

PVY were purified with the procedure including PEG precipitation and differential centrifugation .

将PVY毒原摩擦接种于白肋烟扩繁后，采用PEG沉降及超离心的方法进行病毒的提纯。

Hepatic endoplasmic reticulum was isolated by differential centrifugation with sucrose density gradient .

采用蔗糖密度梯度离心法分离肝脏内质网。

Myocardial mitochondria was extracted by differential centrifugation , and protein level was measured by Coomassie brilliant blue method .

用差速离心法提取心肌线粒体，考马斯亮蓝法测定蛋白含量。

Methods Human recombinant tau protein in vitro was purified by using differential centrifugation , heat inactivation and ion exchange chromatography .

方法依次使用差速离心、热失活、离子交换层析的方法纯化体外重组的人tau蛋白；

Mitochondrial respiratory function and COX activity were measured by Clark oxygen electrode . Mitochondria were obtained by differential centrifugation .

应用差速离心法提取脑皮质线粒体，Clark氧电极法测定线粒体呼吸功能，及细胞色素C氧化酶（COX）的活性。

The virus was purified by means of chloroform clarification , polyethyleneglycol precipitation and differential centrifugation .

用氯仿澄清，聚乙二醇沉淀和差速离心可部分提纯。

You subject the resulting heart homogenate , which is dense and opaque , to a series of differential centrifugation steps .

你把得到的密集而不透明的牛心匀浆液用一系列的差速离心。这是为了什么目的？

The green leaves of vegetables through tissue homogenate , differential centrifugation separation , density gradient centrifugation purify sample of chloroplast .

对蔬菜绿叶通过组织匀浆，差速离心分离，密度梯度离心分离、纯化、提取叶绿体。

The concentration of 8 elements in subcellular fractions of normal human liver has been determined by a combination of differential centrifugation and neutron activation analysis .

将超离心分离技术与中子活化分析法相结合，分析测定了正常人肝亚细胞组分中的微量元素。

Wear particles in the osteolytic tissue can be obtained with strong base digestion and differential centrifugation , which is up to the demand of experimental study .

通过强碱消化及密度梯度离心提取、纯化假体周围骨溶解组织中磨损颗粒的方法可提供符合试验需要的金属颗粒。

Prepared sarcoplasmic reticular ( SR ) of colonic muscle by differential centrifugation and measured Ca 2 + ATPase activity with colorimetric assay .

差速离心制备结肠平滑肌肌浆网，测定肌浆网Ca2+ATP酶活性。

SDS-PAGE and immunoblotting test of Hantavirus ( HTV ) R84-1 strain purified by differential centrifugation were carried out .

对差速离心纯化的汉坦病毒R84-1毒株进行了SDS-PAGE和免疫印迹试验。

The 8-week-old female Balb / c mice were immunized with the allantoic fluid , which was purified and concentrated by differential centrifugation .

病毒经差速离心法纯化后，四次免疫8周龄Balb/c雌性小鼠。

In this study , 252 acidophilic actinomycetes were isolated from 10 acidic soil samples collected in Jiangxi Province , Yunnan Province and Beijing with dispersion and differential centrifugation approach .

实验中，从江西、云南、北京采集的10份酸性土样，采用分散和差速离心法和常规分离方法分离出嗜酸放线菌252株。

Purified preparations of the nucleocapsid of WRSV were obtained by a combination of organic solvent treatment , PEG precipitation , differential centrifugation and isoelectric precipitation .

结合有机溶剂沉淀、聚乙二醇沉淀及差速离心和等电点沉淀等方法可以获得小麦丛矮病毒核衣壳的纯化制剂。

Synaptosomes were isolated from pig brain by homogenization , differential centrifugation and sucrose gradient centrifugation . Study on Bull Sperm Separation by Percoll Density with Centrifuge

以猪脑为材料，经匀浆、差速离心、蔗糖密度梯度离心分离突触体.Percoll密度梯度离心分离牛精子初探

The microsomal fractions were obtained by the method of differential centrifugation , and HO-1 ( Heme Oxygenase-1 ) activity in testicular microsomal was measured .

通过亚细胞分离方法提取大鼠睾丸微粒体，测定微粒体血红素氧化酶-1（HO-1）活性。

The results showed that the concentration efficiency of speed differential centrifugation is superior to that of membrane filteration and the concentration efficiency of speed differential centrifugation speed can attain 34.5 % .

结果显示：差速离心法的浓缩效率要优于滤膜截留法的浓缩效率，其浓缩效率能达到345%。

Methods The distribution pattern of 17 elements in subcellular fractions of human hepatocellular carcinoma was studied by differential centrifugation combined with NAA , then compared to the human liver with t-test .

方法低温差速离心分离肝癌亚细胞组份，应用NAA法检测各亚细胞组份的微量元素含量，然后与正常人肝及癌旁组织的相应亚细胞组份相比较。

The liver microsomal fraction was prepared by differential centrifugation and the activity of liver microsomal enzymes P450 , b5 and aniline hydroxylase in rats were studied .

采用差速离心法制备大鼠肝微粒体，测定细胞色素P450、b5含量及苯胺羟化酶活力。

Its intracellular distribution in myocardium of rabbits has been studied by differential centrifugation . The components of cells were fractionated into three parts , nuclear , mitochondrial and supernatant fraction .

是一个有效抗心律失常药用差离心法研究了该药在家兔心肌亚细胞部位，核、线粒体和上清液三部分的分布。

The differential centrifugation technique and sequential chemical extraction method were used to study the subcellular distribution and the chemical forms of Cu in the roots , leaves and stems of Commelina communis .

运用差速离心法和化学试剂逐步提取法分析了Cu在鸭跖草Commelinacommunis根、茎、叶的亚细胞分布及贮存形态。

Methods : The sepsis was prepared on rat by cecal ligation and puncture . 45 Ca 2 + uptake and release assay were performed on rat liver nuclei isolated by differential centrifugation .

方法：通过结扎并穿刺盲肠制作大鼠败血症模型，差速离心制备细胞核，进行45Ca2+摄取和释放测定。

The mitochondria was extracted from the inner leaves of CMS lines and their maintainer lines by using differential centrifugation and sucrose shelf method , then the mtDNA was extracted by SDS-Proteinase K method .

该方法以大白菜球叶为材料，采用差速离心和蔗糖衬垫相结合的方法分离出线粒体，然后用SDS&蛋白酶K裂解法提取mtDNA。

The purified virions of the isolate TN were obtained by differential centrifugation and stained negatively with 2 % uranyl acetate . The virus particle morphology was spherical about 28 nm in diameter .

病毒粒体为正20面体，直径约25nm.用差速离心等方法提纯的病毒，经醋酸铀负染，粒子为20面体，直径为28nm。

Methods : Based on differential centrifugation and sucrose shelf method , the mitochondria was extracted from cytoplasmic male sterile lines and their maintainer lines of Chinese cabbage and the mtDNA was extracted successfully by SDS-Proteinase K method .

方法：采用差速离心和蔗糖衬垫相结合的方法先从大白菜胞质雄性不育系及保持系叶片中分离出线粒体，然后用SDS&蛋白酶K裂解法提取mtDNA。

Generally it includes isolation of purified nuclei by differential centrifugation , embedment of the nuclei in either low-melting-point agarose plugs or microbeads , and digestion with proteinase K in situ to release high molecular weight ( HMW ) DNA .

该方法利用差速离心分离植物细胞核，经低熔点琼脂糖块或低熔点琼脂糖微珠包埋，蛋白酶K原位裂解后制备大分子量核DNA。